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Use of some exotic natural extracts in cosmetics
Phan, Huong Tra ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The objective of this bachelor thesis was a general characterization of the antioxidant effect of exotic plants and their extracts. The selected plants were Zingiber officinale, Turmeric longa and Momordica charantia. Theoretical part focuses on antioxidative properties, genereal characteristics of selected plants and description of the methods applied in the experimental part. In the experimental part, content of the antioxidant activity, polyphenols, flavonoids were characterized by spectrophotometry. Major active ingredient (curcumin, gingerol) and other substances like tocopherol were determined by HPLC/UV-VIS. Finally, potential practical use of these active substances in the field of cosmetics including skin hydration measurement was tested.
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Příprava receptury bezlepkového chleba obohaceného o vybrané antioxidanty a posouzení jeho senzorické hodnoty
PECHOVÁ, Klára
Bread has been one of the basic foodstuffs for more than 30,000 years. It´s prepared mainly by baking, where gluten plays an important role. Gluten is important for holding the gas in the dough to achieve the desired structure, texture and volume. At a time when agriculture began to develop, a disease of gluten began to emerge. One of the best known is the intolerance of gluten, the so-called celiac disease, which affect sapproximately 1 % of the world's population. The cause is gluten-rich proteins (prolamins and glutelins), found in wheat, rye, barley and oats. The second known diseaseis gluten allergy, which is often confused with celiac disease. The difference consist in a different autoimmune reaction to the presence of glucose, when the immunization on glucose produces IgE immunoglobulin and does not damage the small intestine mucosa The only possible treatment for patiens with a dinase is a strict gluten-free diet. Gluten-free breads and pastries are one of the main foods for celiac and allergy sufferers. Preparing a dough without gluten is difficult. Proper selection of raw materials plays an important role in meeting sensory properties. The aim of the diploma thesis was to prepare suitable recipe senriched with selected sources of antioxidants. In total, six different recipes were prepared, both from cereal gluten-free flour and legumes flour. Curcuma and red onion skin were selected sources of antioxidants. In the practical part a sensory evaluation of all samples of bread is performed. There was a positive evaluation of the questionnaire itself. When to Cheb the suitability of raw materials and procedures for dough preparation and baking. Based on the DPPH and FRAP tests, antioxidant aktivity was evaluated for all gluten-free bread. Bread senriched with red onion skin have the highest antioxidant activity in both basic bread and basic bread with legume flour. Thus, it can be said that onion skin effectively increase antioxidant activity. In the final part, a financial evaluation of all six types of gluten-free bread was made on the basis of raw materials used as compared to those already purchased in the shop. Based on this finding, it can be said that home preparation is a cheaper option.
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Application of Hyb-Seq method for reconstruction of reticulate infrageneric phylogeny: example from polyploid genus Curcuma L. (Zingiberaceae)
Skopalíková, Jana ; Fér, Tomáš (advisor) ; Krak, Karol (referee)
This master thesis focuses on the phylogeny of hybridogenous and polyploid genus Curcuma from family Zingiberaceae using Next-Generation Sequencing data from hundreds to thousands nuclear loci. This approach seems to be better than widely used cpDNA and ITS sequencing especially in the case of hybridogenous and polyploid groups. Data for phylogeny reconstruction were generated using Hybridization-based sequencing (Hyb-Seq) method which combines target enrichment and genome skimming strategies. Data analysis was performed primarily using pipeline HybPhyloMaker especially created for Hyb-Seq data analysis. Twenty-seven species from the genus Curcuma and three outgroup species were sequenced in this work. Phylogenetic trees based on all 1 154 and 811 selected nuclear low- copy genes show high support values of all nodes which is in contrast to plastome and rDNA phylogeny with lower support values in some nodes and incongruences in topology compared to low-copy genes phylogeny. Phylogenetic networks inferred from low-copy genes, lineage movement analysis and monophyly tests agree with published hypotheses of interlineage hybrid origin of three species - C. vamana, C. myanmarensis and C. roscoeana. These analyzes show likely hybrid origin of C. candida too with parents from the group Curcuma I and basal...
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Use of some exotic natural extracts in cosmetics
Phan, Huong Tra ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The objective of this bachelor thesis was a general characterization of the antioxidant effect of exotic plants and their extracts. The selected plants were Zingiber officinale, Turmeric longa and Momordica charantia. Theoretical part focuses on antioxidative properties, genereal characteristics of selected plants and description of the methods applied in the experimental part. In the experimental part, content of the antioxidant activity, polyphenols, flavonoids were characterized by spectrophotometry. Major active ingredient (curcumin, gingerol) and other substances like tocopherol were determined by HPLC/UV-VIS. Finally, potential practical use of these active substances in the field of cosmetics including skin hydration measurement was tested.
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